Coding

Part:BBa_K3753000:Design

Designed by: Jiaying Hu   Group: iGEM21_NJTech_China   (2021-09-18)


PAAS-Petunia


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 60
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding region of petunia PAAS was obtained by PCR amplification using Petunia hybrida genome as template. (Gen-Bank accession number DQ243784.1) OD600

Source

Petunia hybrida cv. Mitchell

References

Kaminaga Yasuhisa et al. Plant Phenylacetaldehyde Synthase Is a Bifunctional Homotetrameric Enzyme That Catalyzes Phenylalanine Decarboxylation and Oxidation*[J]. Journal of Biological Chemistry, 2006, 281(33) : 23357-23366.